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Stomate Experiment

Materials /Method

What did we do?


What I think will happen?

Wind: The plant will lose more water because wind will causes humidity to decrease thus creating a gradiant between the water in plant and the dry air around it. Humidity: The plant will lose less water because of higher water potential outside the plant causing a decrease in transpiration.Light: The plant will lose water at an increase rate because there is constant photosynthesis.

-Water and macronutrients are used to synthesize new molecules -Plants absorb and tranport water through xylem and nutrients through the pholem -Water transport water through the xylem through cohesion, adhesion, and the bonding of hydrogen molecules-Hydrogen bonding is the atraction of polar molecules between a slightly positive hydogen atom and a slightly negative oxygen atom -Water also travels through capillary action were liquid is able to flow through narrow passageways against gravity.-Tonicity osmotic pressure gradient were water flows from high water potential to low water potential. (Campbell, 2007) -For example, in a giant redwood when there is a higher water potential outside the plant otherwise known as humidity there is a decrease of transpiration.-Transpiration is when water evaporates from the leaves after being produced by photosynthesis -Water leaves the plant through the stomata.-Gaurd cells around the stomata control the amount of water leaving the plant(Lab Manual Transpiration, 2012)

1. Take the plant out of the container and wrap the saturated soil around with plastic wrap.2. Cut off the flowers to get the most accurate measurements of the mass3. Flower 1 is the control and flower 2 is the experimental. The experimental plant was placed under the fan for four days.4. The mass was taken for both plants everyday aswell as the percent change.|Final-Initial|/Initial x 100


What happened & Why?

-To find the average amount of stomata on the leaf.-Two leafs were measured on graph paper -Leaf 1 was 800 mm squared -Leaf 2 was 850 mm squared - Nail polish was applied to the bottom of leaf and removed with tape.-Piece of nail polish was put on a slide to see the stomates that were stuck to it and observe under a microscope - It was found that the average number of stomates on both leaves was 111,641.225-The reason the stomates are on the bottom side of the leaf is because the sun is directed to the top of the leaf and this a means to prevent water loss.

By: TeamSAS: Sharon, Alex K, and Sage


-The experiment done supported the wind hypothesis -Due to the increase of wind, the rate of transpiration was faster compared to the control - Wind causes the humidity to drop around the plant - This humidity decrease causes the air around the plant to become drier -By the air being drier outside the plant it causes water to move along its gradient and diffuse outsude the plant-The experiment done supported the humidity hypothesis -Due to the increase of humidity, the rate of transpiration was slower compared to the contol -Humidity is the amount of water vapor in the atmosphere -As humidity increases, so does the water vapor in the atmosphere -This increase in humidity causes there to be more water outside the plant than inside - By there being more water outside the plant, it causes less water to diffuse outside the plant-The experiment done supported the light hypothesis -Due to the increase in light intensity, the rate of transpiration was faster compared to the control - The increase in light intensity causes an increase in photosynthesis - Photosynthesis excretes water as a waste product -By photosynthesis increasing so does water being produce and leaving the plant through the stomata -According to Marc D Abrams, southeasern oaks curl their leaves to reduce transpiration and retain water in arid areas. (Abrams, 1990) -Stomates are placed on the bottom of the leaf to prevent dehydration from evaporating water-As surface area of a leaf increases so does the rate of transpiration. This is because more of the leaf is exposed to the sun

-Flowers-Fan-Plastic wrap-Sissors -Calculator -Scale

-Wind had the greatest rate of change-Light had the second greatest rate of change-Moisture had the least rate of change

Abrams, M. (). Tree Physiology . Adaptations and responses to drought in Quercus species of North America, , 229.Campbell, Neil A., and Jane B. Reece. AP Edition Biology. 7th ed. . San Francisco, California: Pearson Education Inc. , 2005. Print.College Board. (2012). investigation 11 Transpiration. Retrieved from


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