Genetic Engineering

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Last updated 6 years ago

Discipline:
Science
Subject:
Genetics
Grade:
9

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Genetic Engineering

Genetic Engineering

Both LB plates showed a lot of growth while the LB/A plates had no growth at all.

The transformed E. coli with the ampicillin resistance gene will be able to grow in the ampicillin plates, but the non-transformed E. coli will not.

Expected Outcome:

The bacteria treated with the pAMP solution developed a resistance to ampicillin and were able to grow on the ampicillin+ plate. Those that were not treated with the pAMP were not able to grow on this medium. The plates with no ampicillin served as a control to show how the bacteria would look in normal conditions. Transformation is never fully effective, Only cells that are competent enough are able to take up the foreign DNA. Therefore, the ampicillin + plates showed less growth than the control plate.

The plasmid is cut open with a restriction enzyme with in the Betegalactosidase gene. If the DNA ligase inserts the new DNA into the plasmid, the gene for Betegalactosidase is disrupted. When the new plasmid is transformed in to the bacteria, the disrupted version of Betegalactosidase no longer produces the protein that interacts with X-gal to produce blue colonies. Thus, the bacteria that have the plasmid containing the new fragment of DNA will be white.

Plasmid Preparation:

WHY

This pBlu lab had for purpose to transform the strain of E. coli bacteria due to new genetic information being introduced into the cell. The bacteria have to create a clone with a resistance to ampicillin in order to culture. The E. coli transforms the pBlu plasmid, which carries the genes coding for two identifiable phenotypes. If colonies grew in the dish, then the transformation was effective.

I think that genetic engineering can and will be used in the future. But, I only agree that it should be used for preventing life threatening or incurable diseases. Creating a human in a lab that is stronger or smarter than average would only lead to much disagreement and possible disasterous mistakes.

Genetic engineering can heavily impact society in the future. Humans could be modifyed with immunity against diseases like cancer or inhanced athletic ability. It also includes the potential of using engineered bacteria to turn waste in to useful products.

Purpose:


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