Effects of Training

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by CarlaBC
Last updated 5 years ago

Discipline:
Science
Subject:
Cell Biology

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Effects of Training

EFFECTS OF TRAINING AND ACUTE EXERCISE ON MITOCHONDRIAL BIOGENESIS AND ANTIOXIDANT CAPABILITIES IN FOOTBALL PLAYERS’ BLOOD MONONUCLEAR CELLS

Experimental ProcedureTwelve young male federated football players were recruited and performed a program of exercise for 8 weeks consisting of a 2 hours/day football training session. One blood sample was obtained in basal conditions before the training period. After 8 weeks, another two samples were taken, before and after an acute and intense exercise. PBMCs fraction was isolated from fresh blood using Ficoll-Paque Plus and low-speed centrifugation. The isolated suspension of PBMCs was subjected to whole-cell protein extraction for the determination of NF-kB activation, which was performed using an ELISA method. COXIV, MitND5 and PGC1α mRNA expression was determined by multiplex real-time PCR using human 18S rRNA as reference. COXIV, PGC1α, UCP2 and UCP3 protein levels were determined by Western blot.

REFERENCES1.- Ji LL. 1995. Free Radic Biol Med 18: 1079-1086 .2.- Sureda A, Batle JM, Ferrer MD, Mestre-Alfaro A, Tur JA, Pons A. 2012. J Sports Med 33:531-536.3.- Radak Z, Zhao Z, Koltai E, Ohno H, Atalay. 2013. Antioxid Redox Signal 18:1208-1246.4.- Ferrer MD,Sureda A, Mestre A, Tur JA, Pons A. 2010 Cell Physiol Biochem 25: 241-252.5.- Cases N, Aguiló A, Tauler P, Sureda A, Llompart I, Pons A,Tur JA. 2005 Eur J Clin Nutr. 59:781-788 6- Vina J, Borràs C, Góme´-Cabrera MC, Orr WC. 2006. Free Radic Res 40:111-119

RESULTSThe Western Blot results reported a significant increase in antioxidant protein levels - UCP2 and UCP3 – and in COXIV after the 8-week training period in basal conditions. PGC1α and UCP2 protein levels in 8-weeks samples also increased after the acute exercise bout respect to pre-exercise value. After the training period, expression of PGC1 α, COXIV and mitND5 mRNA significantly augmented after the acute physical activity respect to pre-exercise value, while no changes were observed in these mRNA respect pre-training results. NF-kB activation reported a significant increase after the acute exercise bout once the 8-week training finished.


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