Broth Mini-Lab

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by ghadley
Last updated 5 years ago

Discipline:
Science
Subject:
Life Science
Grade:
7

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Broth Mini-Lab

Broth Mini-Lab

Introduction

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Procedure

My Hypothesis

If the fresh broth has no bacteria in it, then Pasteur’s controlled experiment was proven correct because living things cannot arise from nonliving sources.

What does Dirty Broth look like compared to Fresh Broth?

1. Mrs. G opened and left an unsealed container of chicken broth on the counter of the prep room starting on January 3rd2. On Friday, January 9th students will open a fresh new container of chicken broth.3. Students will then exam both samples and compare them.4. Students will note observations, take photos if it is helpful for the comparison.5. Students will compare both samples under the microscope.6. Students will attempt to get a photo of the samples underneath the microscope, they must be sure to note the magnification in order to receive full credit.

Data, Observations, and Analysis

Conclusion

The theory of spontaneous generation is the idea that living things arise from nonliving things. Before Louis Pasteur had proven this theory incorrect, spontaneous generation was believed true. Pasteur conducted an experiment that using “swan necks” that he made. He put fresh broth into two swan necks. The swan necks allowed the magical air in, but kept mold and bacteria out. To kill all the remaining bacteria, he boiled the broth to kill any bacteria that may have gotten in. He waited a year and the broth was still fresh in both swan necks. He then broke off one of the swan necks. Now that one was broken off, it allowed air and bacteria to come in. Within a few days the broken swan neck had bacteria in it. The swan neck that was not broken remained fine. This controlled experiment proved the theory of spontaneous generation incorrect. In our experiment, our hypothesis was: If the fresh broth has no bacteria in it, then Pasteur’s controlled experiment was proven correct because living things cannot arise from nonliving sources. The fresh broth showed no sign of bacteria while looking at it and even under the microscope. It was sealed shut from any bacteria that may had gotten in. While the dirty broth was exposed to all bacteria. The dirty broth did not look or smell good. This is because of the bacteria was able to get to the broth and start living in it. My partner and I found something interesting under the microscope while comparing the fresh broth to the dirty broth. We noticed that the dirty broth had little spots. We saw that these were groupings of bacteria. The fresh broth did not have any spots to it. This lab has helped support my hypothesis by proving that fresh broth does not have any bacteria. This lab is like the Pasteur experiment by proving that the idea of spontaneous generation is incorrect.

Our Question

Spontaneous generation is the idea that living things arise from nonliving sources. This theory was believed by everyone until it was proven incorrect by Louis Pasteur. He conducted an experiment in which he had a controlled experiment. He put Fresh Broth into two glasses with “swan necks”. The swan necks allowed air in, but did not let mold and bacteria in. He then boiled the broth in the glasses to kill all remaining bacteria. He waited a year until he broke the swan neck off of one glass and left one glass with the neck on. No bacteria lived in the broth before. Once one of the swan necks was broken, three days later bacteria started living inside of it. The one with the swan neck still on it remained completely fine. By conducting this controlled experiment, he proved that the idea of spontaneous generation was incorrect.

It is easy to see that the Dirty Broth is very disgusting compared to the Fresh Broth. It is spotted all over with dark brownish spots. That is where bacteria is growing. On the other hand, the Fresh Broth is completely clear of bacteria. The Dirty Broth looks like the remains of a plate of buffalo wings. The Fresh Broth looks like a plate that came straight out of a dishwasher. This controlled experiment also proves that the idea of spontaneous generation is incorrect.

By: George Hadley


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