# Bioluminescence of Dinoflagellates

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Last updated 8 years ago

Discipline:
Science
Subject:
Life Science
12

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Day 3 Trial 7 Trial 8 Trial 9 Measure in Absorbance Units 0.043 0.031 0.032 0.031 0.043 0.035 0.037 0.034 0.055 0.032 0.03 0.031 0.04 0.037 0.032 0.032 0.031 0.033 0.035 0.041 0.039 0.032 0.03 0.033 0.035 0.032 0.05

Bioluminescence of Dinoflagellates

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My Equipment

Method

What did I do?

My Hypothesis

What do I think will happen?

I predict that as if the amount of light exposure is reduced, the luminescence will decrease and, likewise, if the amount of light exposure is increased, the luminescence will increase.

Does the luminescence of Pyrocystis Lunula change based on the amount of light the culture is exposed to?

The Question

Day 2 Trial 4 Trial 5 Trial 6 Measure in Absorbance Units 0.082 0.089 0.037 0.044 0.037 0.052 0.063 0.043 0.041 0.036 0.037 0.036 0.039 0.031 0.038 0.033 0.035 0.037 0.036 0.036 0.037 0.037 0.034 0.047 0.03 0.043 0.039

Prelab note: Place Pyrocystis Lunula cultures into a Erlenmeyer flask and place under flourescent lighting, turning the light on from 5 p.m to 5 a.m in order to feed the culture.1. Begin the experiment by exposing the cultures to the flourescent light.2. At a time increment of thirty minutes, use a pipette to fill three cuvettes with culture samples. Take these three samples from three different regions of the cylinder.3. Place a stopper on the cuvettes and agitate one sample by inverting the cuvette three times, then place the agitated sample in the spectrometer and record the flourescence reading in Absorbance Units (AU), then place samples back in cylinder.4. Repeat process for the remaining two cuvettes then place samples back in the culture.5. Repeat steps 2-4 for a total of 240 minutes (3 hours).

Results

What happened?

Day 1Minutes Trial 1 Trial 2 Trial 3 Measure in Absorbance Units0 0.032 0.033 0.03530 0.041 0.03 0.03360 0.04 0.053 0.04192 0.032 0.033 0.04120 0.036 0.038 0.034150 0.031 0.041 0.036180 0.034 0.038 0.036210 0.045 0.043 0.033240 0.037 0.042 0.037

The results of my experiment contrasted my hypothesis. Rather than the fluorescence of the Pyrocystis lunula culture rising linearly with the amount of light it was exposed to, the fluorescence stayed relatively similar and even fluctuated (Figure 2). This test simply demonstrates that cultures of Pyrocystis lunula shine with the same intensity regardless of fluorescent light exposure time. However, If this study was performed with a different species of bioluminescent dinoflagellate, the effects of luciferin binding proteins (LBP) could be tested.

Conclusion