Bacterial Transformation

Genetically Engineering E.coli Genes:

Abstract

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Our Results

My hypothesis

What will happen?

Method

Please refer to the materials and procedures found in the AP Biology Investigative Labs Manual (College Board, 2012), Investigation 8 - Biotechnology: Bacterial Transformation.................................................................................................................................................................................................................................................................................................

One of the many purposes served by plasmids, is the transfer of genes between organisms to acquire specific traits. In this lab, E.coli genes are manipulated by inserting a bioluminescent gene from jellyfish, to create a glowing E.coli bacteria. The purpose of this lab is to genetically engineer E.coli to display a trait of our choice. Plasmids allow manipulation of genetic information, and makes it possible to transfer DNA easily between bacterium, along with arabinose sugar, this effort can easily be achieved.

The purpose of this lab is to genetically engineer E.coli to display the glow in the dark characteristic found in jellyfish. Extracting DNA from one organism and inserting it into another is a powerful tool of biotechnlogy; which can enable scientists to transfer desirable genes into virtually any organism. This lab report presents the procedure, the experiment's results, and an analysis of the results.

Null Hypothesis: The E.coli sample will not acquire glow in the dark properties from the bioluminscent jellyfish gene.Alternative Hypothesis: The E.coli sample will acquire glow in the dark properties from the bioluminscent jellyfish gene.Prediction: The E.coli sample will acquire the glow in the dark.

Introduction